In Ovo Injection of Triaminohexanoic Acid in Fowl: Gross Investigation

Objective: In recent years, amino acids are used in veterinary medicine for many purposes. They injected into hatching eggs to increase chick body weight and performance at hatch, but the adverse effects of amino acids have always been a major concern. There is scant information available about the pathological alterations of amino acids in the chicken embryonated eggs. The objective of this study was to investigate the pathological alterations of triaminohexanoic acid for chicken embryo. Methods: Fertile chicken eggs were divided into two equal treatment groups as follows: phosphate buffered saline-injected group and triaminohexanoic-injected group whose individuals were injected with a triaminohexanoic acid solution at a dosage of 10 mg per Kg egg-weight. Embryos were re-incubated post-treatment and allowed to develop until day 18 after which; they were examined for macroscopic lesions. Results: Results showed that embryos were normal in all treatment groups. Macroscopically, no lesions were also diagnosed in tissues. Based on macroscopic findings, it is concluded that triaminohexanoic acid at above-mentioned concentration is not toxic for the chicken embryo. Therefore, triaminohexanoic acid egg-injection can be used for increasing chick performance at hatch without any adverse effect.